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KMID : 0545120050150010197
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 1 p.197 ~ p.201
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Statistical Optimization of Chitinase Production by Pantoea dispersa to Enhance Degradation of Crustacean Chitin Waste
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Vipul G
Jiwan D/Vyas P/Chhatpar HS
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Abstract
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A novel chitinase-producing bacterial strain of Pantoea dispersa was isolated from the sea near Bhavnagar India for efficient disposal of chitinous waste from the seafood processing industry. The medium components were optimized by using a cubic model in the central composite design for increasing chitinase production. The optimal concentrations for higher production of chitinase were (g l-1) chitin 10.0; urea 0.35; MgSO4 7H2O 0.08 and CaCl2 0.15. Here peptone (0.05 g l-1) was used as a constant variant in all trials. Using a statistical optimization method the chitinase production was found to increase from 108 to 486.4 units ml-1. Chitin was prepared from the crustacean waste and Fourier Transform Infrared (FTIR) Spectroscopy was used to characterize the isolated chitin. Chitinous waste degradation was studied in terms of chitinase production.
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